Microplate Readers and Reader Accessories
Microplate readers are a type of spectrophotometer designed specifically for analyzing multiple samples in multi-well plates.
The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. The signals are produced by, converted by, or transmitted through the samples and are measured by a detector. The detectors are usually photomultiplier tubes (PMT), which convert the released photons into electricity. The current is quantified by the reader’s software to produce the numerical result.
In some cases, samples are first excited by exposure to light of specific wavelengths. Filters or monochromators are used to select or restrict the wavelengths needed for emission or detection.
Single-mode microplate readers commonly measure absorbance, fluorescence, or luminescence. Multi-mode plate readers may measure absorbance, fluorescence, and luminescence with options for fluorescence polarization (FP). They may also include lasers for time-resolved fluorescence (TRF), TR-FRET, Amplified Luminescent Proximity Homogeneous Assays (ALPHA), and cell imaging.
Features may include:
- Monochromator- or filter-based wavelength selection
- Visible only or UV/Vis spectrum, fluorescence, and luminescence
- Multiple photomultiplier tubes (PMTs) for simultaneous assays
- Temperature and CO2/O2controls
- Modular design for adding functionality
Microplate readers are used for high-throughput screening (HTS), biomarker assays, nucleic acid and protein quantification, kinetics, ELISA assays, homogeneous time resolved fluorescence (HTRF) for measuring cytokines, bioluminescence resonance energy transfer (BRET), and other techniques.
Revvity Health Sciences Inc HTRF SARS-Cov2 Spike S1 protein detection kit, 500 Assay Points
HTRF SARS-Cov2 Spike S1 protein detection kit, 500 Assay Points
Revvity Health Sciences Inc HTRF Human IL-23 / IL23R Binding Kit Standard
HTRF Human IL-23 / IL23R Binding Kit Standard
Molecular Devices Absorbance Filter, 640 nm, 8 nm BW
The AD 340 absorbance photometric filter provides a narrowband 640 nm passband for absorbance measurements in filter-wheel microplate readers. It is supplied as a single replacement filter for compatible readers and supports assays that require red-wavelength detection.
- Absorbance detection at 640 nm center wavelength.
- Bandwidth 8 nm.
- Designed for filter-wheel microplate reader configurations.
- Single replacement filter for maintenance and assay reproducibility.
- Supports red-wavelength absorbance assays and endpoint measurements.
Molecular Devices Photometric Filter, Absorbance 590 nm, FilterMax Compatible
The AD 340 photometric filter provides a 590 nm center wavelength bandpass for absorbance (photometric) measurements in multimode microplate readers. Supplied as an accessory by Molecular Devices (MPN 6590-0023), the filter installs in a reader's filter holder to enable targeted optical selection for absorbance assays and routine photometric measurements.
- Central wavelength 590 nm for absorbance measurements.
- Designed for photometric microplate assays.
- Installs in a standard filter holder for quick replacement.
- Provides consistent optical selection for repeatable readings.
Molecular Devices Fluorescence Polarization Filter, 535 nm, 25 nm, Vertical
Fluorescence polarization filter optimized for fluorescence polarization assays. The filter has a 535 nm center wavelength with a 25 nm bandwidth and a vertical polarization orientation. It includes a holder for mounting in compatible microplate readers and is intended for use with FP measurement configurations.
- Optimized for fluorescence polarization measurements.
- Center wavelength 535 nm, bandwidth 25 nm.
- Vertical polarization orientation for polarization-based assays.
- Supplied with a holder for secure mounting in reader filter positions.
- Compatible with SpectraMax and similar microplate readers that accept filter holders.
Molecular Devices Absorbance Filter, 260 nm, 12 nm Bandwidth
The Molecular Devices FilterMax absorbance filter provides selective UV detection at 260 nm with a 12 nm bandwidth for accurate absorbance measurements on compatible multi-mode microplate readers. It is a replacement optical filter intended for absorbance assays such as nucleic acid quantification and related UV measurements.
- Center wavelength 260 nm for UV absorbance measurements.
- 12 nm bandwidth (full width at half maximum) for narrow spectral selection.
- Designed for use in multi-mode microplate readers with removable filter slides.
- Sold individually as a single replacement filter.
- Supports accurate nucleic acid and protein absorbance assays.
Perkin Elmer US LLC Split/Splitless Injector Starter Kit for Manual Gas Control
Includes the following pieces: (5) Ultra Inert Splitless Liners with Wool 4 mm I.D. (09200696) (5) Ultra Deactivated Split/Splitless Liners with Glass Wool, 4 mm I.D. (09200624) (10) Silicone O-Rings (09200714) (50) Green Injection Port Septa (N6621028) (10) 0.4 mm (09200685), (10) 0.5 mm (09200686) and (10) 0.8 mm Graphite/Vespel Ferrules (09200687) (2) Gold Seal Washers (N6551043) (1) Gold Seal Nut (N6552080) (1) Capillary Injector Nut (N6552084) (1) Ceramic Column Cutter (N9301376)
Revvity Health Sciences Inc HTRF Human Androgen Receptor Full Length Detection Kit, 10,000 Assay Points
HTRF Human Androgen Receptor Full Length Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human Androgen Receptor Variant 7, Control Lysate
HTRF Human Androgen Receptor Variant 7, Control Lysate
Revvity Health Sciences Inc Tag-lite pSNAP-HTR4 Serotonin Receptor Plasmid, 10 µg
Tag-lite pSNAP-HTR4 Serotonin Receptor Plasmid, 10 µg
Revvity Health Sciences Inc HTRF dsRNA (IVT) Detection Kit, 500 Assay Points
HTRF dsRNA (IVT) Detection Kit, 500 Assay Points